Selected Publications
Theoretical Analysis of Cell Separation Based on Cell Surface Marker
Density
Chalmers, J.J, Mandal, S., Fang, B., Sun, L., Zborowski, M., Biotechnology
and Bioengineering 59:10-20 (1998).
Continuous cell separation using novel magnetic quadrupole flow sorter
Zborowski, M., Sun. L., Moore, L.R., Williams, P.S., Chalmers, J.J.
J. of Magnetism and Magnetic Materials, 194:224-230 (1999).
An Instrument to determine the magnetophoretic mobility of paramagnetic
particles and labeled, biological cells.
Chalmers, J.J., Zhao, Y., Nakamura, M., Melnik, K. Lasky, L., Moore,
L., Zborowski, M., . J. of Magnetism and Magnetic Materials. 194:231-241
(1999).
Hydrodynamic Properties in Bioreactors
Chattopadyay, D., M. Garcia-Briones, R. Venkat, J.J. Chalmers, In: Mammalian
Cell Biotechnology in Protein Production, Eds: H. Hauser, R. Wagner,
Walter de Gruyter Publishers, Berlin, (1997)
Study of Hydrodynamics in Microcarrier Culture Spinner Vessels: A
Particle Tracking Velocimetry Approach
Venkat, R., R. Stock, and J.J. Chalmers, Biotechnology and Bioengineering
49:456-466, (1996).
A Thermodynamic Explanation for Cell Adhesion to Gas-Liquid Interfaces
Chattopadhyay, D., J. Rathman, and J.J. Chalmers, Biotechnology and
Bioengineering Vol. 48 p.649-658 (1995).
Quantification of Suspended Cell Damage as a Result of Bubble Rupture
Trinh, K., M. Garcia-Briones, F. Hink and J.J. Chalmers, Biotechnology
and Bioengineering, Vol. 43, p. 37-45 (1994).
Computer Simulations of the Ruptureof a Gas Bubble at a Gas-Liquid
Interface and its Implications in Animal Cell Damage
Garcia-Briones, M.A., R.S. Brodkey, J.J. Chalmers, Chemical Engineering
Science, Vol. 49:2301-2320 (1994).
U.S. Patents
Method for magnetically separating cells into fractionated flow streams
Zborowski, M., Chalemrs, J., Moore, L. US Patent # 5,968,820; 1999
Method for Determining Particle Characteristics,
Chalmers, J.J., Zborowski, M., Moore, L. U.S. Patent # 5,974,901;
1999
System and Device for Determining Particle Characteristics
Chalmers, J.J., Zborowski, M., Moore, L. U.S. Patent # 6,082,205;
2000
Fractional cell sorter,
Zborowski, M., Chalmers, J.J. Moore, L. U.S. Patent 6,120,735, 2000.
Method for determining particle characteristics,
Zborowski, M., Chalmers, J.J. Moore, L. U.S. Patent 6,142,025, 2000.
System and Device for determining particle characterisitics,
Zborowski, M.,Chalmers, J.J., Moore, L., U.S. Patent 6,412,359, 2002.
Complete List of Publications
Antibody saturation studies of three antigens proposed for use in
rare cancer cell detection.
Chosy, J., Melnik, K., Comella, K., Zborowski, M., Chalmers, J.J.
Biotechnology and Bioengineering 82:340-351. 2003.
Red Blood Cell Magnetophoresis,
Zborowski, M., Ostera, G.R., Moore, L.R., Milliron, S., Chalmers, J.J.,
Schechter, A.N. Biophysics Journal 84:2638-2645. 2003.
Splitter Imperfections in Annular Split-Flow Thin Channel Separations
of Cells: Effect of Non-Specific Crossover.
Williams, S., Moore, L.R., Chalmers, J.J., Zborowski, M. Analytical
Chemistry 75(6):1365-1373. 2003.
The Frabrication and Use of A Transient Contractional Flow Device
to Quantify the Sensitivity of Mammalian and Insect Cells to Hydrodynamic
Forces
Ma, Ningning, Koelling, K., Chalmers, J.J.Biotechnology and Bioengineering
80:428-437. 2002. Erratum 81:379. 2003.
Separations based on magnetophoretic mobility.
Zborowski, M., Moore, L.R., Williams, S. Chalmers, J.J., Separation
Sciences and Technology, 37: 3611-3633. 2002.
Pulse-injection studies of blood progenitor cells in a quadrupole
magnetic flow sorter.
Hoyos, M., McCloskey, K., Moore, L., Nakmura, M., Bolwell, B., Chalmers,
J., Zborowski, M. J. Separation Science and Technology 37(4) 1-23. 2002.
Sparation of Breast Cancer Cells by Quadrupole Magnetic Flow Sorter.
Nakamura, M., Decker, K., Chosy, J., Comella, K., Melnik, K. Moore,
L., Zborowski, M., Chalmers, J.J. Biotechnology.Progress 17:1145-1155.
2001.
The effect of antibody concentration on the separation of human natural
killer cells in a commercial immuno-magnetic separation system.
Comella, K., Melnik, K., Chosy, J., Zborowski, M., Cooper, M.A., Fehniger,
T.A. Caligiuri, M.A., and Chalmers, J.J. Cytometry 45:285-293. 2001.
Evaluation of eluants from batch separations of CD34(+) cells from
human cord blood using a commercial, immunomagnetic cell separation system.
Melnik, K. Nakamura, M., Comella, K., Zborowski, M., Chalmers, J.J.
Biotechnol Progress 17(5):907-916.2001.
Mobility measurements of immunomagnetically labeled cells allow quantitation
of secondary antibody binding amplification.
McCloskey, K., Comella,K., Margel, S., Chalmers, J., Zborowski, M.
Biotechnol. Bioeng. 75:642-655. 2001.
Study of magnetic particles pulsed-injected into an annular SPLITT-like channel inside a quadrupole magnetic field, Hoyos, M., Moore, L.R., McCloskey, K.E., Margel S., Zuberi, M., Chalmers, J.J., Zborowski, M. J. Chromatogr., A , 903(1-2):99-116. 2000.
Measurement of CD2 Expression Levels of IFN-Treated Fibrosarcomas
Using Cell Tracking Velocimetry,
McCloskey, K., Chalmers, J.J., Zborowski, M., Cytometry. Jun 1; 44(2):137-47.
2001.
Progenitor cell isolation with a high-capacity quadrupole magnetic
flow sorter,
Moore, L.R., Rodrigues, A.R., Williams, P.S., McCloskey, K., Bolwell,
B.J., Nakamura, M., Chalmers, J.J. J. Magn. Magn. Mater. 225(1-2):277-284.
2001
Theoretical and Experimental Analysis of the Accuracy of Cell Tracking
Velocimetry
Nakamura, N., Lasky, L., Zborowski, M., Chalmers, J.J. Experiments
in Fluids 30:371-380. 2001.
Magnetic FFF
Zborowski, M., Williams, S, Chalmers, J.J., Encyclopedia of Chromatography,
Marcel Dekker, Inc. New York, 2001. Pp.503-507.
The use of Magnetite-Doped Polymeric Microspheres in Calibrating Cell
Tracking Velocimetry,
Moore, L.R. Sun, L.P., Zborowski, M., Nakamura, M., Chalmers, J.J.,
McCloskey, K. Gura, S., Burdygin, I., Margel, S., .J. Biochemical and
Biophysical Methods 44:115-130, 2000.
Magnetophoretic Mobilities Correlate to Antibody Binding Capacity
McCloskey, K. E., Chalmers, J.J, Zborowski, M. Cytometry 40:307-315.
2000.
Cell damage of microcarrier and suspended cell culture as a function
of extensional forces and local energy dissipation
Gregoriades, N., Clay, J., Koelling, K., Chalmers, J. Biotechnology
and Bioengineering 69:171-183, 2000.
Cell-Microcarrier adhesion to Gas-Liquid Interfaces and Foam
Bauer, T., Hancock, L., Rathman, J., Chalmers, J.J. Biotechnology
Progress 16:125-132, 2000.
Flow Rate for the Quadrupole Magentic Cell Sorter
Williams, P.S.,Zborowski, M., Chalmers, J.J., Analytical Chemistry
71:3799-3807, 1999.
Rapid cell isolation by magnetic flow sorting for applications in
tissue engineering
Zborowski, M., Sun, L., Moore, L.R., Chalmers, J.J. , ASIO Journal
45:127-130, 1999.
Quantification of Cellular Properties from External Fields and Resulting
Induced Velocity: Cellular Hydrodynamic Diameter.
Chalmers, J.J., Haam,S., Zhou, Y., McCloskey, K., Moore, L., Zborowski,
M., Williams, P.S., Biotechnology and Bioengineering 64:509-518. (1999)
Quantification of Cellular Properties from External Fields and Resulting
Induced Velocity: Magnetic Susceptibility,
Chalmers, J.J., Haam, S.,Zhou, Y., McCloskey, K., Moore, L., Zborowski,
M., Williams, P.S. Biotechnology and Bioengineering 64:519- 526. (1999)
Lymphocyte fractionation using immunomagnetic colloid and dipole magnet
flow cell sorter
Moore, L.R., Zborowski, M., Sun, L. Chalmers, J.J., J. Biochemical
and Biophysical Methods. 37:11-33. (1998)
Continuous, Flow-Through Immunomagnetic Cell Separation in a Quadrupole
Field
Sun, L., Zborowski, M., Chalmers, J.J., Cytometry. 33:469-475. (1998).
Characterization of the Degradation of Poly- Lactic Acid in a Well-Controlled
Composting System
Agarwal, M., Koelling, K., Chalmers, J.J., Biotechnology Progress,
14:517-526, (1998).
Immunomagnetic Cell Separation
Chalmers, J., Sun, L., Moore, L., Zborowski, M. , Biotechnology Progress,
14:141-148, (1998).
Gas Bubbles and their Influence on Microorganisms,
Chalmers, J.J., Applied Mechanics Reviews, 51: 113-121, (1998)
A Linked Supercritical Extraction- Biodegradation System to Extract
and Degrade Phenol
Wheeler, G., D. Tomasko, J.J. Chalmers, Chemical Engineering Science,
53:189-201. (1998)
A Confocal Microscopic Observation of Non-Submerged Biofilms
Chalmers, J.J., Vir, R., Biotechnology Progress, 13:727-732 (1997)
Continuous-flow Magnetic Cell Sorting Using Soluble Immunomagnetic
Labels
M. Zborowski, L.R. Moore, L. Sun, J.J. Chalmers, In: Scientific and
Clinical Applications of Magnetic Microcarriers, Eds: W. Schuett, U. Hafeli,
J. Teller, M. Zborowski, Plenum Press, (1997)
Cylindrical Split and Quadrupole Magnetic Field in Applications to
Continuous-Flow Magnetic Cell Sorting
Zborowski, M., P.S. Williams, L. Sun, L. R. Moore, J.J. Chalmers, J.
Liquid Chromatography and Related Techniques, 20:2887-2905. (1997)
Shear sensitvity of insect cells
J.J. Chalmers, Cytotechnology 20:163-171, (1996)
Characterization of agitation environments in 250 ml spinner vessel,
3 L, and 20 L reactor vessel used for animal cell microcarrier culture
Venkat, R.V., J.J. Chalmers, Cytotechnology 22:95-102. (1996)
A Fourier-Transform Infrared Spectroscopic Analysis of Organic Matter
Degradation in a Bench-Scale Solid Substrate Fermentation (Composting) System
Tseng, D.Y., R. Vir, S.J. Traina, J. J. Chalmers, Biotechnology and
Bioengineering 52:661-671. (1996)
ATP measurements in Compost
Tseng, D.Y., O.H. Tuovinen, and J.J. Chalmers, Compost Science and
Utilization, Vol. 4:6-17, (1996).
Immunomagnetic Identification of Human Lymphocytes Using Magnetoferritin
Zborowski, M., C.B. Fuh, R. Green, N.J. Baldwin, S. Reddy, T. Douglas,
S. Mann, J. J. Chalmers, Cytometry Vol. 24:251-259, (1996).
Determination of the Magnetic Susceptibility of Labelled Particles
by Video Imaging
Reddy, S., L. Moore, M. Zborowski, J. J. Chalmers, Chemical Engineering
Science, Vol. 51:947-956, (1996).
Shear Sensitivity of Insect Cells
Chalmers, J.J., In: Insect Cell Cultures: Fundamental and Applied
Aspects, Just Vlak, et al.(eds)., Kluwer Academic Publishers, (1996).
Analytical Magnetopheresis of Ferritin-labeled Lymphocytes
Zborowski, M., C. B. Fuh, R. Green, L. Sun, J.J. Chalmers, Analytical
Chemistry, Vol. 67 p.3702-3712 (1995).
Characterization of a Bench-Scale System for Studying the Biodegradation
of Organic Solid Wastes
Tseng, D.Y., O.H. Tuovinen, H.A.J. Hoitink, and J.J. Chalmers, Biotechnology
Progress, 11(4):443-451. (1995)
The Protective Effect of Specific Medium Additives with Respect to
Bubble Rupture
Chattopadhyay, D., Rathman, J., J.J. Chalmers, Biotechnology and
Bioengineering, Vol. 45 p.473-480 (1995).
The Effect of Hydrodynamic Forces on Insect Cells
Chalmers, J. J.,, In: Insect Cell Culture: Production of Improved
Biopesticides and Proteins From Recombinant DNA, Michael Shuler, (ed.) Hanser
Publishers, New York, (1995).
Cells and Bubbles in Sparged Bioreactors
Chalmers, J.J., Cytotechnology, Vol. 15, p. 311-320 (1994)
Flow Parameters Associated with Hydrodynamic Cell Injury
Garcia-Briones, J.J. Chalmers, Biotechnology and Bioengineering,
Vol. 44 p. 1089-1098 (1994).
Cell-Bubble Interactions: Mechanisms ofSuspended Cell Damage
Garcia-Briones, M.A., and J.J. Chalmers, Annals of the New York Academy
of Sciences, Vol. 665:219-229 (1992).
Microscopic Visualization of Insect Cell-Bubble Interactions I: Rising
Bubbles, Air-Medium, and the Foam Layer
Bavarian, F., L.S. Fan, J.J. Chalmers, Biotechnology Progress,Vol. 7, No.
2, p. 140-150 (1991).
Microscopic Visualization of Insect Cell-Bubble Interactions II: The
Bubble Film and Bubble Rupture
Chalmers, J.J., F. Bavarian, Biotechnology Progress, Vol. 7, No. 2, 151-158(1991).
Glucose-stat: A Predictive and Feedback ControlledContinuous Culture
Kleman, G., J.J. Chalmers, W. Strohl, Applied and Environmental Microbiology,
Vol. 57, No. 4, 918-923(1991).
Predictive and Feedback Control Provides ConstantGlucose Concentration
in Fed-batch Fermentations
Kleman, G., J.J. Chalmers, W. Strohl, Applied and EnvironmentalMicrobiology,
Vol. 57, No. 4, 910-917 (1991).
The Protective Effect of Methylcelluloseon Suspended Insect Cells
Subjected to Laminar Shear Stress
Goldblum, S.D., Y.K. Bae, W. Hink J. Chalmers, Biotechnology Progress, 6:383-390
(1990).
A Simple Membrane Reactor to Obtain High Cell Density and Plasmid Encoded Protein Production from Escherichia coli, Chalmers, J.J., Shuler, M.L., Wilson, D.B., Biotechnology Techniques, 4:73-78 (1990).
Effects of Temperature on the Stability of Escherichia coli Overproducing -lactamase or Human Epidermal Growth Factor, Chalmers, J.J., Kim, E., Telford, J.N., Shuler, M.L., Wilson, D.B., Applied and Environmental Microbiology, 56:104-111 (1990).
Continuous Immobilized Recombinant Protein Production from E. coli Capable of Selective Protein Excretion: A Feasibility Study, Georgiou, G., Chalmers, J.J., Shuler, M.L., Wilson, D.B., Biotechnology Progress, 1:75-79 (1985).